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healthy crl 2097  (ATCC)


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    ATCC healthy crl 2097
    Healthy Crl 2097, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/healthy+crl+2097/pm41781386-328-19-17?v=ATCC
    Average 96 stars, based on 333 article reviews
    healthy crl 2097 - by Bioz Stars, 2026-08
    96/100 stars

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    Confirmation of the expression of the newly identified gene, IRX2 , in neuronal cells containing dopaminergic neurons. ( A ) Characterization of neuronal cells containing dopaminergic neurons from healthy control (WT), LRRK2 G2019S familial (GS), and sporadic (SPD) PSCs. The neuronal marker (Microtubule-associated protein 2; MAP2) and the dopaminergic neuronal marker (Tyrosine hydroxylase; TH) were co-stained (scale bar 20 μm). ( B ) Relative expression levels of IRX2 (WT, 0.7100 ± 0.2900, n = 2; PD, 1.598 ± 0.1473, n = 5, p -value 0.0281) in familial/sporadic PD patient-derived neuronal cells containing dopaminergic neurons (PD) in comparison with normal control-derived cells (WT). ( C ) Determination of IRX2 in human <t>fibroblast</t> cells. Examination of IRX2 (WT, 1.033 ± 0.2582, n = 8; PD, 2.047 ± 0.3931, n = 8, p -value 0.0489) expression in familial/sporadic PD patient-derived fibroblast (PD) and healthy control fibroblast (WT) through qRT-PCR analysis. Data are mean ± standard error of means (SEM) of at least three independent experiments. p -values were analyzed using the unpaired two-tailed Student’s t-test (* p < 0.05).
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    IC 50 values of S. tetragona extracts estimated in the MTT cytotoxicity assay.

    Journal: Saudi Pharmaceutical Journal : SPJ

    Article Title: Phytochemical profiling of Salsola tetragona Delile by LC-HR/MS and investigation of the antioxidant, anti-inflammatory, cytotoxic, antibacterial and anti-SARS-CoV-2 activities

    doi: 10.1016/j.jsps.2023.101731

    Figure Lengend Snippet: IC 50 values of S. tetragona extracts estimated in the MTT cytotoxicity assay.

    Article Snippet: Human healthy skin fibroblasts CCD-1079Sk (CRL-2097TM) and human breast cancer cells MCF7 (HTB-22TM) were acquired from American Type Culture Collection (ATCC, VA, USA).

    Techniques:

    Confirmation of the expression of the newly identified gene, IRX2 , in neuronal cells containing dopaminergic neurons. ( A ) Characterization of neuronal cells containing dopaminergic neurons from healthy control (WT), LRRK2 G2019S familial (GS), and sporadic (SPD) PSCs. The neuronal marker (Microtubule-associated protein 2; MAP2) and the dopaminergic neuronal marker (Tyrosine hydroxylase; TH) were co-stained (scale bar 20 μm). ( B ) Relative expression levels of IRX2 (WT, 0.7100 ± 0.2900, n = 2; PD, 1.598 ± 0.1473, n = 5, p -value 0.0281) in familial/sporadic PD patient-derived neuronal cells containing dopaminergic neurons (PD) in comparison with normal control-derived cells (WT). ( C ) Determination of IRX2 in human fibroblast cells. Examination of IRX2 (WT, 1.033 ± 0.2582, n = 8; PD, 2.047 ± 0.3931, n = 8, p -value 0.0489) expression in familial/sporadic PD patient-derived fibroblast (PD) and healthy control fibroblast (WT) through qRT-PCR analysis. Data are mean ± standard error of means (SEM) of at least three independent experiments. p -values were analyzed using the unpaired two-tailed Student’s t-test (* p < 0.05).

    Journal: International Journal of Molecular Sciences

    Article Title: Iroquois Homeobox Protein 2 Identified as a Potential Biomarker for Parkinson’s Disease

    doi: 10.3390/ijms21103455

    Figure Lengend Snippet: Confirmation of the expression of the newly identified gene, IRX2 , in neuronal cells containing dopaminergic neurons. ( A ) Characterization of neuronal cells containing dopaminergic neurons from healthy control (WT), LRRK2 G2019S familial (GS), and sporadic (SPD) PSCs. The neuronal marker (Microtubule-associated protein 2; MAP2) and the dopaminergic neuronal marker (Tyrosine hydroxylase; TH) were co-stained (scale bar 20 μm). ( B ) Relative expression levels of IRX2 (WT, 0.7100 ± 0.2900, n = 2; PD, 1.598 ± 0.1473, n = 5, p -value 0.0281) in familial/sporadic PD patient-derived neuronal cells containing dopaminergic neurons (PD) in comparison with normal control-derived cells (WT). ( C ) Determination of IRX2 in human fibroblast cells. Examination of IRX2 (WT, 1.033 ± 0.2582, n = 8; PD, 2.047 ± 0.3931, n = 8, p -value 0.0489) expression in familial/sporadic PD patient-derived fibroblast (PD) and healthy control fibroblast (WT) through qRT-PCR analysis. Data are mean ± standard error of means (SEM) of at least three independent experiments. p -values were analyzed using the unpaired two-tailed Student’s t-test (* p < 0.05).

    Article Snippet: Healthy fibroblasts (IMR90, MRC5, and CRL-2097) were purchased from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Expressing, Control, Marker, Staining, Derivative Assay, Comparison, Quantitative RT-PCR, Two Tailed Test